This project is designed for someone aged 12 years or older.

Click here for instructions in PDF

Quickstart Guide:

How Do I Get a Sample?

To take a water sample, you will need a bottle (at least 150 mL) and a Sharpie pen (for labelling the bottle). Wash the bottle out with some soap and then rinse with water until there is no soap left in the bottle (rinse at least 3 times). Once you have what you need, you can head to your local stream or other water source.

The most important rule about taking water samples is to stay safe:

  • Don’t go out of your comfort zone
  • Take a friend with you
  • Use a stick to hold your bottle so you don’t have to go too deep

If you are sampling from a stream, try to:

  1. Take the sample from a place where the water flows and mixes freely.
  2. If you can, take samples from a place with a record of water quality measurements so you can compare your measurements against the record.

If you are sampling from a bay, try to:

  1. Take three samples: one from the middle and one from each end of the bay
  2. Take your sample from a middle depth between the bottom and the surface

When you’re getting your water sample, rinse the bottle out with the water from the water source first. This will wash out any leftover soap or water from washing the bottle because we don’t want them to interfere with our measurements later on. Then you can fill the bottle with water from the source.

What to Do?

This is a quick way to estimate the amount of nitrate in a water sample. With a bit of practice, this test will take you about 15 minutes to do.

1.     Turn on Te Kete Waiora at least 10 minutes before use. This gives the LED the chance to “warm-up” fully.

 

2.     Put 5 mL of the water sample into a clean and empty plastic vial.

 

 

3.     Shake up API nitrate test bottle #1 and bottle #2 for 15 seconds

 

4.     To the plastic vial: add 10 drops from API nitrate test bottle #1 then 10 drops from bottle #2

 

5.     Close the vial’s lid tightly so it doesn’t spill

6.     Shake the plastic vial for 30 seconds

 

7.     Let it rest for at least 5 minutes to give the chemical reaction time to change colour

 

 

While you’re waiting:

 

8.     Add the standard solution provided with your test kit to the 50 mL flask. Fill the 50 mL flask with the sample until it reaches the line marked on the flask. This is the spiked sample.

 

9.     Measure 5 mL of the spiked sample into another plastic vial

 

 

  1. Repeat steps 3. to 7. for this vial.

Before you measure:

  1. Take out your two cuvettes
  2. Fill one of your cuvettes using the water sample you are testing. This is your reference cell.
  3. Keep the other cuvette for the samples

Measuring the concentration using Te Kete Waiora:

  1. Use “UP”/“DOWN” buttons to find the option called “Concentration” in the main menu. Press “RIGHT” to choose this mode.
  2. Using “UP”/“DOWN” and “RIGHT”, change the number shown to match the concentration of the standard solution you were given. (Check the bottle the standard solution comes in.)
  3. Press “SELECT” to move to the next screen.
  4. Using “UP”/“DOWN” and “RIGHT”, set the ratio to 0.04 (because we add 2 mL of standard solution to make a 50 mL spiked sample, 2/50 = 0.04).
  5. Press “SELECT”.
  6. Follow the instructions on screen to finish your measurement.

After your measurement

  1. The device will tell you the concentration of the sample. Record your results in your notebook.
  2. If you would like to confirm this value, you can repeat steps 2. to 7. and 9. to 18. 4 more times and take the average result as the concentration of the sample.
  3. Clean any vials, flasks, bottles and cuvettes you used with soap before rinsing the soap out with water (at least 3 times). Keep these for next time.

How it Works

This test relies on colour change to measure the amount of nitrate in a sample. The change is caused by a reaction between chemicals we add from our API test kits and the nitrate in the water sample.

We use a colorimeter to measure the intensity of the colour change. The colourimeter lets us use a number to describe “how red” something is and makes it easier to tell the difference between two samples that look similar.

Measuring Nitrate:

We spike our water sample by adding a standard solution containing a known amount of nitrate. This makes the solution more red. The difference in redness between the sample and the spiked sample allows us to work out the amount of nitrate in the water sample. We need this comparison because the colour change depends a lot on what else is in the water. For example: in salt water, the colour usually changes less than the same amount of nitrate in fresh water.